.svg)
Camila Hochman Mendez, MSc, PhD
Dr. Camila Hochman-Mendez is the Texas Heart Institute’s Assistant Director of Regenerative Medicine Research and Director of the Biorepository and Cell Profiling Lab. Dr. Hochman-Mendez’s research centers on the role of extracellular matrix proteins on cardiac stem cell differentiation, tissue repair, and regeneration.
Show full bioDr. Hochman-Mendez received her Ph.D. in Biophysics from the Federal University of Rio de Janeiro (UFRJ). She completed a Postdoctoral Research Fellowship in Bioengineering from the Universitat Politecnica de Valencia in Spain, followed by a series of Postdoctoral Research Fellowships in tissue engineering research programs at the Institute of Biophysics Carlos Chagas Filho in Brazil. Prior to joining THI in 2017, Dr. Hochman-Mendez’s research at the Federal University of Rio de Janeiro, Brazil, culminated in the first report characterizing the fractal dimension of a biomimetic version of laminin polymers (PolyLM) and several studies on PolyLM’s antifibrotic effects in iPSCs and small preclinical models.
As Director of the THI Biorepository and Cell Profiling Lab, Dr. Hochman-Mendez oversees a College of American Pathologists (CAP)-accredited biorepository core facility providing storage of critical biospecimens and performing phenotypic and function analyses to clinical investigators in the Texas Medical Center and nationwide. For the past eight years, the THI Biorepository and Cell Profiling Lab have served as a biorepository core facility to the NHLBI Cardiovascular Cell Therapy Research Network (CCTRN) and the Cardiothoracic Surgical Trials Network (CTSN).
As Assistant Director of the Regenerative Medicine Research department, Dr. Hochman-Mendez’s research program focuses on four areas:
1. Engineering of in vitro and ex vivo cardiovascular tissue models utilizing a combination of cells, biologically active molecules, and innate biological components.
2. Optimization of extracellular matrix-based culture systems for efficient induced pluripotent stem cell (iPSC) expansion and directed cardiomyocyte differentiation.
3. Development of a closed bioreactor system integrating mechanical and electrical stimulation and automated critical parameter monitoring for recellularizing whole organ scaffolds.
4. Investigations targeting basement membrane proteins and the regulation of their gene expression in cardiovascular development and disease pathogenesis.
Hide full bio
Texas Heart Institute Positions
- Assistant Investigator; Director, THI Biorepository and Cell Profiling Lab
- Assistant Investigator; Assistant Director, Regenerative Medicine Research
Honors, Awards and Memberships
Publications
4862227
MJL5PY3A
1
alternatives-to-animal-experimentation
10
date
desc
Hochman-Mendez
1936
https://www.texasheart.org/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22NIQV76E3%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Chacon-Alberty%20et%20al.%22%2C%22parsedDate%22%3A%222023-01-17%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BChacon-Alberty%2C%20L.%2C%20Fernandez%2C%20R.%2C%20Jindra%2C%20P.%20et%20al.%20%282023%29.%20Primary%20graft%20dysfunction%20in%20lung%20transplantation%3A%20a%20review%20of%20mechanisms%20and%20future%20applications.%20%26lt%3Bi%26gt%3BTransplantation%26lt%3B%5C%2Fi%26gt%3B.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1097%5C%2FTP.0000000000004503%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1097%5C%2FTP.0000000000004503%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Primary%20graft%20dysfunction%20in%20lung%20transplantation%3A%20a%20review%20of%20mechanisms%20and%20future%20applications%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lourdes%22%2C%22lastName%22%3A%22Chacon-Alberty%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ramiro%22%2C%22lastName%22%3A%22Fernandez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Jindra%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Madelyn%22%2C%22lastName%22%3A%22King%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ivan%22%2C%22lastName%22%3A%22Rosas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camila%22%2C%22lastName%22%3A%22Hochman-Mendez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gabriel%22%2C%22lastName%22%3A%22Loor%22%7D%5D%2C%22abstractNote%22%3A%22Lung%20allograft%20recipients%20have%20worse%20survival%20than%20all%20other%20solid%20organ%20transplant%20recipients%2C%20largely%20because%20of%20primary%20graft%20dysfunction%20%28PGD%29%2C%20a%20major%20form%20of%20acute%20lung%20injury%20affecting%20a%20third%20of%20lung%20recipients%20within%20the%20first%2072%20h%20after%20transplant.%20PGD%20is%20the%20clinical%20manifestation%20of%20ischemia-reperfusion%20injury%20and%20represents%20the%20predominate%20cause%20of%20early%20morbidity%20and%20mortality.%20Despite%20PGD%26%23039%3Bs%20impact%20on%20lung%20transplant%20outcomes%2C%20no%20targeted%20therapies%20are%20currently%20available%3B%20hence%2C%20care%20remains%20supportive%20and%20largely%20ineffective.%20This%20review%20focuses%20on%20molecular%20and%20innate%20immune%20mechanisms%20of%20ischemia-reperfusion%20injury%20leading%20to%20PGD.%20We%20also%20discuss%20novel%20research%20aimed%20at%20discovering%20biomarkers%20that%20could%20better%20predict%20PGD%20and%20potential%20targeted%20interventions%20that%20may%20improve%20outcomes%20in%20lung%20transplantation.%22%2C%22date%22%3A%22Jan%2017%2C%202023%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1097%5C%2FTP.0000000000004503%22%2C%22ISSN%22%3A%221534-6080%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22GNSKWJNU%22%2C%22MJL5PY3A%22%2C%22UDYH8SFB%22%2C%22JD5ZU4IS%22%5D%2C%22dateModified%22%3A%222023-03-14T14%3A35%3A47Z%22%7D%7D%2C%7B%22key%22%3A%2269GRKEEX%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Mesquita%20et%20al.%22%2C%22parsedDate%22%3A%222022-12-07%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BMesquita%2C%20F.%20C.%20P.%2C%20Leite%2C%20E.%20S.%2C%20Morrissey%2C%20J.%20et%20al.%20%282022%29.%20Polymerized%20laminin-521%3A%20a%20feasible%20substrate%20for%20expanding%20induced%20pluripotent%20stem%20cells%20at%20a%20low%20protein%20concentration.%20%26lt%3Bi%26gt%3BCells%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bi%26gt%3B11%26lt%3B%5C%2Fi%26gt%3B%2C%203955.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcells11243955%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcells11243955%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Polymerized%20laminin-521%3A%20a%20feasible%20substrate%20for%20expanding%20induced%20pluripotent%20stem%20cells%20at%20a%20low%20protein%20concentration%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fernanda%20C.%20P.%22%2C%22lastName%22%3A%22Mesquita%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eliel%20S.%22%2C%22lastName%22%3A%22Leite%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jacquelynn%22%2C%22lastName%22%3A%22Morrissey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Catarina%22%2C%22lastName%22%3A%22Freitas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tatiana%22%2C%22lastName%22%3A%22Coelho-Sampaio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camila%22%2C%22lastName%22%3A%22Hochman-Mendez%22%7D%5D%2C%22abstractNote%22%3A%22Laminins%20%28LNs%29%20play%20a%20central%20role%20in%20the%20self-assembly%20and%20maintenance%20of%20basement%20membranes%20and%20are%20involved%20in%20critical%20interactions%20between%20cells%20and%20other%20extracellular%20matrix%20%28ECM%29%20proteins.%20Among%20the%20defined%2C%20xeno-free%20ECM%20culture%20matrices%2C%20LNs-namely%20LN521-have%20emerged%20as%20promising%20coating%20systems%20for%20the%20large-scale%20expansion%20of%20induced%20pluripotent%20stem%20cells%20%28iPSCs%29.%20The%20biologic%20activity%20of%20LNs%20is%20enhanced%20by%20their%20acidification-induced%20self-polymerization%20into%20a%20cell-associated%20network%20called%20polylaminin%20%28polyLN%29%2C%20which%20can%20recapitulate%20the%20native-like%20polymeric%20array%20in%20a%20cell-free%20system.%20Here%2C%20we%20show%20for%20the%20first%20time%20to%20our%20knowledge%20that%20polyLN521%20displays%20a%20native-like%20hexagonal-like%20structure%20and%20that%2C%20at%20basal%20and%20low%20concentrations%2C%20it%20permits%20the%20large-scale%20expansion%20of%20human%20iPSCs.%20Human%20iPSCs%20expanded%20with%20polyLN521%20maintained%20the%20pluripotent%20state%20and%20showed%20no%20impairment%20of%20karyotype%20stability%20or%20telomere%20length.%20These%20results%20suggest%20that%20low-concentration%20polyLN521%20is%20a%20stable%20and%20cost-effective%20coating%20for%20large-scale%20iPSC%20expansion.%22%2C%22date%22%3A%22Dec%2007%2C%202022%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.3390%5C%2Fcells11243955%22%2C%22ISSN%22%3A%222073-4409%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MJL5PY3A%22%2C%22PXVTG4ES%22%2C%22N9FF4AWY%22%2C%22TXW6RMW4%22%5D%2C%22dateModified%22%3A%222023-01-06T17%3A14%3A44Z%22%7D%7D%2C%7B%22key%22%3A%22K3ZBBBHU%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Chacon-Alberty%20et%20al.%22%2C%22parsedDate%22%3A%222022-11-29%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BChacon-Alberty%2C%20L.%2C%20Ye%2C%20S.%2C%20Elsenousi%2C%20A.%20et%20al.%20%282022%29.%20Effect%20of%20intraoperative%20support%20mode%20on%20circulating%20inflammatory%20biomarkers%20after%20lung%20transplantation%20surgery.%20%26lt%3Bi%26gt%3BArtif%20Organs%26lt%3B%5C%2Fi%26gt%3B.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Faor.14474%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Faor.14474%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Effect%20of%20intraoperative%20support%20mode%20on%20circulating%20inflammatory%20biomarkers%20after%20lung%20transplantation%20surgery%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lourdes%22%2C%22lastName%22%3A%22Chacon-Alberty%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shengbin%22%2C%22lastName%22%3A%22Ye%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Abdussalam%22%2C%22lastName%22%3A%22Elsenousi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emma%22%2C%22lastName%22%3A%22Hills%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Madelyn%22%2C%22lastName%22%3A%22King%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ethan%22%2C%22lastName%22%3A%22D%27Silva%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andres%20Pena%22%2C%22lastName%22%3A%22Leon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marcelo%22%2C%22lastName%22%3A%22Salan-Gomez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Meng%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camila%22%2C%22lastName%22%3A%22Hochman-Mendez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gabriel%22%2C%22lastName%22%3A%22Loor%22%7D%5D%2C%22abstractNote%22%3A%22BACKGROUND%3A%20Processes%20that%20activate%20the%20immune%20system%20during%20lung%20transplantation%20can%20lead%20to%20primary%20graft%20dysfunction%20%28PGD%29%20or%20allograft%20rejection.%5CnMETHODS%3A%20We%20analyzed%20cytokine%20expression%20profiles%20after%20reperfusion%20and%20allograft%20outcomes%20in%20a%20cohort%20of%20patients%20%28n%5Cu00a0%3D%5Cu00a059%29%20who%20underwent%20lung%20transplantation%20off-pump%20%28n%5Cu00a0%3D%5Cu00a026%29%2C%20with%20cardiopulmonary%20bypass%20%28CPB%3B%20n%5Cu00a0%3D%5Cu00a018%29%2C%20or%20with%20extracorporeal%20membrane%20oxygenation%20%28ECMO%3B%20n%5Cu00a0%3D%5Cu00a015%29.%20Peripheral%20blood%20was%20collected%20from%20patients%20at%20baseline%20and%20at%206%20and%2072%5Cu00a0h%20after%20reperfusion.%20To%20adjust%20for%20clinical%20differences%20between%20groups%2C%20we%20utilized%20a%20linear%20mixed%20model%20with%20overlap%20weighting.%5CnRESULTS%3A%20PGD3%20was%20present%20at%2048%20or%2072%5Cu00a0h%20after%20reperfusion%20in%207.7%25%20%282%5C%2F26%29%20of%20off-pump%20cases%2C%2020.0%25%20%283%5C%2F15%29%20of%20ECMO%20cases%2C%20and%2038.9%25%20%287%5C%2F18%29%20of%20CPB%20cases%20%28p%5Cu00a0%3D%5Cu00a00.04%29.%20The%20ECMO%20and%20CPB%20groups%20had%20greater%20reperfusion-induced%20increases%20in%20MIP-1B%2C%20IL-6%2C%20IL-8%2C%20IL-9%2C%20IL1-ra%2C%20TNF-alpha%2C%20RANTES%2C%20eotaxin%2C%20IP-10%2C%20and%20MCP-1%20levels%20than%20the%20off-pump%20group.%20Cytokine%20expression%20profiles%20after%20reperfusion%20were%20not%20significantly%20different%20between%20ECMO%20and%20CPB%20groups.%5CnCONCLUSION%3A%20Our%20data%20suggest%20that%2C%20compared%20with%20an%20off-pump%20approach%2C%20the%20intraoperative%20use%20of%20ECMO%20or%20CPB%20during%20lung%20transplantation%20is%20associated%20with%20greater%20reperfusion-induced%20cytokine%20release%20and%20graft%20injury.%22%2C%22date%22%3A%22Nov%2029%2C%202022%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1111%5C%2Faor.14474%22%2C%22ISSN%22%3A%221525-1594%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22GNSKWJNU%22%2C%22MJL5PY3A%22%2C%22UDYH8SFB%22%2C%22TXW6RMW4%22%2C%22AZFR6V65%22%5D%2C%22dateModified%22%3A%222023-01-06T17%3A13%3A34Z%22%7D%7D%2C%7B%22key%22%3A%225FM49INS%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Guan%20et%20al.%22%2C%22parsedDate%22%3A%222022-11-28%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BGuan%2C%20Y.-S.%2C%20Ershad%2C%20F.%2C%20Rao%2C%20Z.%20et%20al.%20%282022%29.%20Elastic%20electronics%20based%20on%20micromesh-structured%20rubbery%20semiconductor%20films.%20%26lt%3Bi%26gt%3BNat%20Electron%26lt%3B%5C%2Fi%26gt%3B.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41928-022-00874-z%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41928-022-00874-z%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Elastic%20electronics%20based%20on%20micromesh-structured%20rubbery%20semiconductor%20films%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ying-Shi%22%2C%22lastName%22%3A%22Guan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Faheem%22%2C%22lastName%22%3A%22Ershad%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhoulyu%22%2C%22lastName%22%3A%22Rao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhifan%22%2C%22lastName%22%3A%22Ke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ernesto%20Curty%22%2C%22lastName%22%3A%22da%20Costa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Qian%22%2C%22lastName%22%3A%22Xiang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yuntao%22%2C%22lastName%22%3A%22Lu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xu%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jianguo%22%2C%22lastName%22%3A%22Mei%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Vanderslice%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camila%22%2C%22lastName%22%3A%22Hochman-Mendez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cunjiang%22%2C%22lastName%22%3A%22Yu%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%22Nov%2028%202022%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41928-022-00874-z%22%2C%22ISSN%22%3A%222520-1131%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41928-022-00874-z%22%2C%22collections%22%3A%5B%22BBMMGS3D%22%2C%22MJL5PY3A%22%2C%229T6HXJUI%22%2C%2235JIYMYN%22%2C%22QF52JASW%22%5D%2C%22dateModified%22%3A%222022-12-01T19%3A16%3A58Z%22%7D%7D%2C%7B%22key%22%3A%22KUR55T7L%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Chacon-Alberty%20et%20al.%22%2C%22parsedDate%22%3A%222022-09-27%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BChacon-Alberty%2C%20L.%2C%20Kanchi%2C%20R.%20S.%2C%20Ye%2C%20S.%20et%20al.%20%282022%29.%20Plasma%20protein%20biomarkers%20for%20primary%20graft%20dysfunction%20after%20lung%20transplantation%3A%20a%20single-center%20cohort%20analysis.%20%26lt%3Bi%26gt%3BSci%20Rep%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bi%26gt%3B12%26lt%3B%5C%2Fi%26gt%3B%2C%2016137.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-022-20085-y%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-022-20085-y%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Plasma%20protein%20biomarkers%20for%20primary%20graft%20dysfunction%20after%20lung%20transplantation%3A%20a%20single-center%20cohort%20analysis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lourdes%22%2C%22lastName%22%3A%22Chacon-Alberty%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rupa%20S.%22%2C%22lastName%22%3A%22Kanchi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shengbin%22%2C%22lastName%22%3A%22Ye%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camila%22%2C%22lastName%22%3A%22Hochman-Mendez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daoud%22%2C%22lastName%22%3A%22Daoud%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cristian%22%2C%22lastName%22%3A%22Coarfa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Meng%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sandra%20L.%22%2C%22lastName%22%3A%22Grimm%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maher%22%2C%22lastName%22%3A%22Baz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ivan%22%2C%22lastName%22%3A%22Rosas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gabriel%22%2C%22lastName%22%3A%22Loor%22%7D%5D%2C%22abstractNote%22%3A%22The%20clinical%20use%20of%20circulating%20biomarkers%20for%20primary%20graft%20dysfunction%20%28PGD%29%20after%20lung%20transplantation%20has%20been%20limited.%20In%20a%20prospective%20single-center%20cohort%2C%20we%20examined%20the%20use%20of%20plasma%20protein%20biomarkers%20as%20indicators%20of%20PGD%20severity%20and%20duration%20after%20lung%20transplantation.%20The%20study%20comprised%2040%20consecutive%20lung%20transplant%20patients%20who%20consented%20to%20blood%20sample%20collection%20immediately%20pretransplant%20and%20at%206%2C%2024%2C%2048%2C%20and%2072%5Cu00a0h%20after%20lung%20transplant.%20An%20expert%20grader%20determined%20the%20severity%20and%20duration%20of%20PGD%20and%20scored%20PGD%20at%20T0%20%286%5Cu00a0h%20after%20reperfusion%29%2C%20T24%2C%20T48%2C%20and%20T72%20h%20post-reperfusion%20using%20the%202016%20ISHLT%20consensus%20guidelines.%20A%20bead-based%20multiplex%20assay%20was%20used%20to%20measure%2027%20plasma%20proteins%20including%20cytokines%2C%20growth%20factors%2C%20and%20chemokines.%20Enzyme-linked%20immunoassay%20was%20used%20to%20measure%20cell%20injury%20markers%20including%20M30%2C%20M65%2C%20soluble%20receptor%20of%20advanced%20glycation%20end-products%20%28sRAGE%29%2C%20and%20plasminogen%20activator%20inhibitor-1%20%28PAI-1%29.%20A%20pairwise%20comparisons%20analysis%20was%20used%20to%20assess%20differences%20in%20protein%20levels%20between%20PGD%20severity%20scores%20%281%2C%202%2C%20and%203%29%20at%20T0%2C%20T24%2C%20T48%2C%20and%20T72%20h.%20Sensitivity%20and%20temporal%20analyses%20were%20used%20to%20explore%20the%20association%20of%20protein%20expression%20patterns%20and%20PGD3%20at%20T48-72%5Cu00a0h%20%28the%20most%20severe%2C%20persistent%20form%20of%20PGD%29.%20We%20used%20the%20Benjamini-Hochberg%20method%20to%20adjust%20for%20multiple%20testing.%20Of%20the%2040%20patients%2C%2022%20%2855%25%29%20had%20PGD3%20at%20some%20point%20post-transplant%20from%20T0%20to%20T72%20h%3B%2012%20%2830%25%29%20had%20PGD3%20at%20T48-72%5Cu00a0h.%20In%20the%20pairwise%20comparison%2C%20we%20identified%20a%20robust%20plasma%20protein%20expression%20signature%20for%20PGD%20severity.%20In%20the%20sensitivity%20analysis%2C%20using%20a%20linear%20model%20for%20microarray%20data%2C%20we%20found%20that%20differential%20perioperative%20expression%20of%20IP-10%2C%20MIP1B%2C%20RANTES%2C%20IL-8%2C%20IL-1Ra%2C%20G-CSF%2C%20and%20PDGF-BB%20correlated%20with%20PGD3%20development%20at%20T48-72%5Cu00a0h%20%28FDR%5Cu2009%26lt%3B%5Cu20090.1%20and%20p%5Cu2009%26lt%3B%5Cu20090.05%29.%20In%20the%20temporal%20analysis%2C%20using%20linear%20mixed%20modeling%20with%20overlap%20weighting%2C%20we%20identified%20unique%20protein%20patterns%20in%20patients%20who%20did%20or%20did%20not%20develop%20PGD3%20at%20T48-72%5Cu00a0h.%20Our%20findings%20suggest%20that%20unique%20inflammatory%20protein%20expression%20patterns%20may%20be%20informative%20of%20PGD%20severity%20and%20duration.%20PGD%20biomarker%20panels%20may%20improve%20early%20detection%20of%20PGD%2C%20predict%20its%20clinical%20course%2C%20and%20help%20monitor%20treatment%20efficacy%20in%20the%20current%20era%20of%20lung%20transplantation.%22%2C%22date%22%3A%22Sept%2027%2C%202022%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-022-20085-y%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22GNSKWJNU%22%2C%22MJL5PY3A%22%2C%22UDYH8SFB%22%2C%22MDTREPAF%22%5D%2C%22dateModified%22%3A%222022-11-04T15%3A26%3A10Z%22%7D%7D%2C%7B%22key%22%3A%22FHWXI2QZ%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Morrissey%20et%20al.%22%2C%22parsedDate%22%3A%222022-06-30%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BMorrissey%2C%20J.%2C%20Mesquita%2C%20F.%20C.%20P.%2C%20Chacon-Alberty%2C%20L.%20et%20al.%20%282022%29.%20Mining%20the%20mesenchymal%20stromal%20cell%20secretome%20in%20patients%20with%20chronic%20left%20ventricular%20dysfunction.%20%26lt%3Bi%26gt%3BCells%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bi%26gt%3B11%26lt%3B%5C%2Fi%26gt%3B%2C%202092.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcells11132092%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcells11132092%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Mining%20the%20mesenchymal%20stromal%20cell%20secretome%20in%20patients%20with%20chronic%20left%20ventricular%20dysfunction%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jacquelynn%22%2C%22lastName%22%3A%22Morrissey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fernanda%20C.%20P.%22%2C%22lastName%22%3A%22Mesquita%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lourdes%22%2C%22lastName%22%3A%22Chacon-Alberty%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camila%22%2C%22lastName%22%3A%22Hochman-Mendez%22%7D%5D%2C%22abstractNote%22%3A%22Close%20examination%20of%20the%20initial%20results%20of%20cardiovascular%20cell%20therapy%20clinical%20trials%20indicates%20the%20importance%20of%20patient-specific%20differences%20on%20outcomes%20and%20the%20need%20to%20optimize%20or%20customize%20cell%20therapies.%20The%20fields%20of%20regenerative%20medicine%20and%20cell%20therapy%20have%20transitioned%20from%20using%20heterogeneous%20bone%20marrow%20mononuclear%20cells%20%28BMMNCs%29%20to%20mesenchymal%20stromal%20cells%20%28MSCs%29%2C%20which%20are%20believed%20to%20elicit%20benefits%20through%20paracrine%20activity.%20Here%2C%20we%20examined%20MSCs%20from%20the%20BMMNCs%20of%20heart%20failure%20patients%20enrolled%20in%20the%20FOCUS-CCTRN%20trial.%20We%20sought%20to%20identify%20differences%20in%20MSCs%20between%20patients%20who%20improved%20and%20those%20who%20declined%20in%20heart%20function%2C%20regardless%20of%20treatment%20received.%20Although%20we%20did%20not%20observe%20differences%20in%20the%20cell%20profile%20of%20MSCs%20between%20groups%2C%20we%20did%20find%20significant%20differences%20in%20the%20MSC%20secretome%20profile%20between%20patients%20who%20improved%20or%20declined.%20We%20conclude%20that%20%26quot%3Bmining%26quot%3B%20the%20MSC%20secretome%20may%20provide%20clues%20to%20better%20understand%20the%20impact%20of%20patient%20characteristics%20on%20outcomes%20after%20cell%20therapy%20and%20this%20knowledge%20can%20inform%20future%20cell%20therapy%20trials.%22%2C%22date%22%3A%22June%2030%202022%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.3390%5C%2Fcells11132092%22%2C%22ISSN%22%3A%222073-4409%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MJL5PY3A%22%2C%22PXVTG4ES%22%2C%22N9FF4AWY%22%2C%22UDYH8SFB%22%2C%222RCWVZ4F%22%5D%2C%22dateModified%22%3A%222022-08-02T14%3A41%3A34Z%22%7D%7D%2C%7B%22key%22%3A%22UMQPRFFZ%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Chacon-Alberty%20et%20al.%22%2C%22parsedDate%22%3A%222021-12-22%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BChacon-Alberty%2C%20L.%2C%20Ye%2C%20S.%2C%20Daoud%2C%20D.%20et%20al.%20%282021%29.%20Analysis%20of%20sex-based%20differences%20in%20clinical%20and%20molecular%20responses%20to%20ischemia%20reperfusion%20after%20lung%20transplantation.%20%26lt%3Bi%26gt%3BRespir%20Res%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bi%26gt%3B22%26lt%3B%5C%2Fi%26gt%3B%2C%20318.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs12931-021-01900-y%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs12931-021-01900-y%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Analysis%20of%20sex-based%20differences%20in%20clinical%20and%20molecular%20responses%20to%20ischemia%20reperfusion%20after%20lung%20transplantation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lourdes%22%2C%22lastName%22%3A%22Chacon-Alberty%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shengbin%22%2C%22lastName%22%3A%22Ye%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daoud%22%2C%22lastName%22%3A%22Daoud%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22William%20C.%22%2C%22lastName%22%3A%22Frankel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hassan%22%2C%22lastName%22%3A%22Virk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jonathan%22%2C%22lastName%22%3A%22Mase%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camila%22%2C%22lastName%22%3A%22Hochman-Mendez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Meng%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Luiz%20C.%22%2C%22lastName%22%3A%22Sampaio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Doris%20A.%22%2C%22lastName%22%3A%22Taylor%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gabriel%22%2C%22lastName%22%3A%22Loor%22%7D%5D%2C%22abstractNote%22%3A%22BACKGROUND%3A%20Sex%20and%20hormones%20influence%20immune%20responses%20to%20ischemia%20reperfusion%20%28IR%29%20and%20could%2C%20therefore%2C%20cause%20sex-related%20differences%20in%20lung%20transplantation%20%28LTx%29%20outcomes.%20We%20compared%20men%26%23039%3Bs%20and%20women%26%23039%3Bs%20clinical%20and%20molecular%20responses%20to%20post-LTx%20IR.%5CnMETHODS%3A%20In%20203%20LTx%20patients%2C%20we%20used%20the%202016%20International%20Society%20for%20Heart%20and%20Lung%20Transplantation%20guidelines%20to%20score%20primary%20graft%20dysfunction%20%28PGD%29.%20In%20a%20subgroup%20of%2040%20patients%20with%20blood%20samples%20collected%20before%20LTx%20%28T0%29%20and%206%2C%2024%2C%2048%20%28T48%29%2C%20and%2072%5Cu00a0h%20%28T72%29%20after%20lung%20reperfusion%2C%20molecular%20response%20to%20IR%20was%20examined%20through%20serial%20analysis%20of%20circulating%20cytokine%20expression.%5CnRESULTS%3A%20After%20adjustment%2C%20women%20had%20less%20grade%203%20PGD%20than%20men%20at%20T48%2C%20but%20not%20at%20T72.%20PGD%20grade%20decreased%20from%20T0%20to%20T72%20more%20often%20in%20women%20than%20men.%20The%20evolution%20of%20PGD%20%28the%20difference%20in%20mean%20PGD%20between%20T72%20and%20T0%29%20was%20greater%20in%20men.%20However%2C%20the%20evolution%20of%20IL-2%2C%20IL-7%2C%20IL-17a%2C%20and%20basic%20fibroblast%20growth%20factor%20levels%20was%20more%20often%20sustained%20throughout%20the%2072%5Cu00a0h%20in%20women.%20In%20the%20full%20cohort%2C%20we%20noted%20no%20sex%20differences%20in%20secondary%20clinical%20outcomes%2C%20but%20women%20had%20significantly%20lower%20peak%20lactate%20levels%20than%20men%20across%20the%2072%5Cu00a0h.%5CnCONCLUSIONS%3A%20Men%20and%20women%20differ%20in%20the%20evolution%20of%20PGD%20and%20cytokine%20secretion%20after%20LTx%3A%20Women%20have%20a%20more%20sustained%20proinflammatory%20response%20than%20men%20despite%20a%20greater%20reduction%20in%20PGD%20over%20time.%20This%20interaction%20between%20cytokine%20and%20PGD%20responses%20warrants%20investigation.%20Additionally%2C%20there%20may%20be%20important%20sex-related%20differences%20that%20could%20be%20used%20to%20tailor%20treatment%20during%20or%20after%20transplantation.%22%2C%22date%22%3A%22Dec%2022%202021%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1186%5C%2Fs12931-021-01900-y%22%2C%22ISSN%22%3A%221465-993X%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22LPUKMNGT%22%2C%22GNSKWJNU%22%2C%223YV626VF%22%2C%22MJL5PY3A%22%2C%22UDYH8SFB%22%2C%22AUPQENLZ%22%5D%2C%22dateModified%22%3A%222022-01-07T20%3A33%3A10Z%22%7D%7D%2C%7B%22key%22%3A%22E3P9LW55%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Mesquita%20et%20al.%22%2C%22parsedDate%22%3A%222021-12-21%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BMesquita%2C%20F.%20C.%20P.%2C%20Morrissey%2C%20J.%2C%20Monnerat%2C%20G.%20et%20al.%20%282021%29.%20Decellularized%20extracellular%20matrix%20powder%20accelerates%20metabolic%20maturation%20at%20early%20stages%20of%20cardiac%20differentiation%20in%20human%20induced%20pluripotent%20stem%20cell-derived%20cardiomyocytes.%20%26lt%3Bi%26gt%3BCells%20Tissues%20Organs%26lt%3B%5C%2Fi%26gt%3B.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1159%5C%2F000521580%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1159%5C%2F000521580%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Decellularized%20extracellular%20matrix%20powder%20accelerates%20metabolic%20maturation%20at%20early%20stages%20of%20cardiac%20differentiation%20in%20human%20induced%20pluripotent%20stem%20cell-derived%20cardiomyocytes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fernanda%20C.%20P.%22%2C%22lastName%22%3A%22Mesquita%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jacquelynn%22%2C%22lastName%22%3A%22Morrissey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gustavo%22%2C%22lastName%22%3A%22Monnerat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gilberto%20B.%22%2C%22lastName%22%3A%22Domont%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fabio%20C.%20S.%22%2C%22lastName%22%3A%22Nogueira%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camila%22%2C%22lastName%22%3A%22Hochman-Mendez%22%7D%5D%2C%22abstractNote%22%3A%22During%20fetal%20development%2C%20cardiomyocytes%20switch%20from%20glycolysis%20to%20oxidative%20metabolism%20to%20sustain%20the%20energy%20requirements%20of%20functional%20cells.%20State-of-the-art%20cardiac%20differentiation%20protocols%20yield%20phenotypically%20immature%20cardiomyocytes%2C%20and%20common%20methods%20to%20improve%20metabolic%20maturation%20require%20multistep%20protocols%20to%20induce%20maturation%20only%20after%20cardiac%20specification%20is%20completed.%20Here%2C%20we%20describe%20a%20maturation%20method%20using%20ventricle-derived%20decellularized%20extracellular%20matrix%20%28dECM%29%20that%20promoted%20early-stage%20metabolic%20maturation%20of%20cardiomyocytes%20differentiated%20from%20human%20induced%20pluripotent%20stem%20cells%20%28hiPSCs%29.%20Chemically%20and%20architecturally%20preserved%20particles%20%2845-500%20%5Cu00b5m%29%20of%20pig%20ventricular%20dECM%20were%20added%20to%20hiPSCs%20at%20the%20start%20of%20differentiation.%20At%20the%20end%20of%20our%20maturation%20protocol%20%28day%2015%20of%20cardiac%20differentiation%29%2C%20we%20observed%20an%20intimate%20interaction%20between%20cardiomyocytes%20and%20dECM%20particles%20without%20impairment%20of%20cardiac%20differentiation%20efficiency%20%28~70%25%20of%20cTNT%2B%29.%20Compared%20with%20control%20cells%20%28those%20cultured%20without%20pig%20dECM%29%2C%2015-day-old%20dECM-treated%20cardiomyocytes%20demonstrated%20increased%20expression%20of%20markers%20related%20to%20cardiac%20metabolic%20maturation%2C%20MAPK1%2C%20FOXO1%2C%20and%20FOXO3%2C%20and%20a%20switch%20from%20ITGA6%20%28the%20immature%20integrin%20isoform%29%20to%20ITGA3%20and%20ITGA7%20%28those%20present%20in%20adult%20cardiomyocytes%29.%20Electrical%20parameters%20and%20responsiveness%20to%20dobutamine%20also%20improved%20in%20pig%20ventricular%20dECM-treated%20cells.%20Extending%20the%20culture%20time%20to%2030%20days%2C%20we%20observed%20a%20switch%20from%20glucose%20to%20fatty%20acid%20metabolism%2C%20indicated%20by%20decreased%20glucose%20uptake%20and%20increased%20fatty%20acid%20consumption%20in%20cells%20cultured%20with%20dECM.%20Together%2C%20these%20data%20suggest%20that%20dECM%20contains%20endogenous%20cues%20that%20enable%20metabolic%20maturation%20of%20hiPSC-CMs%20at%20early%20stages%20of%20cardiac%20differentiation.%22%2C%22date%22%3A%222021-12-21%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1159%5C%2F000521580%22%2C%22ISSN%22%3A%221422-6421%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22MJL5PY3A%22%2C%22PXVTG4ES%22%2C%22N9FF4AWY%22%2C%22IEPEFS59%22%5D%2C%22dateModified%22%3A%222022-02-04T16%3A53%3A46Z%22%7D%7D%2C%7B%22key%22%3A%222DGD6D6X%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hochman-Mendez%20et%20al.%22%2C%22parsedDate%22%3A%222021-12-20%22%2C%22numChildren%22%3A3%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3B%26lt%3Bstrong%26gt%3BHochman-Mendez%26lt%3B%5C%2Fstrong%26gt%3B%2C%20C.%2C%20Mesquita%2C%20F.%20C.%20P.%2C%20Morrissey%2C%20J.%20et%20al.%20%282021%29.%20Restoring%20anatomical%20complexity%20of%20a%20left%20ventricle%20wall%20as%20a%20step%20toward%20bioengineering%20a%20human%20heart%20with%20human%20induced%20pluripotent%20stem%20cell-derived%20cardiac%20cells.%20%26lt%3Bi%26gt%3BActa%20Biomater%26lt%3B%5C%2Fi%26gt%3B%2C%20S1742-7061%2821%2900823%26%23x2013%3B0.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.actbio.2021.12.016%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.actbio.2021.12.016%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Restoring%20anatomical%20complexity%20of%20a%20left%20ventricle%20wall%20as%20a%20step%20toward%20bioengineering%20a%20human%20heart%20with%20human%20induced%20pluripotent%20stem%20cell-derived%20cardiac%20cells%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camila%22%2C%22lastName%22%3A%22Hochman-Mendez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fernanda%20C.%20P.%22%2C%22lastName%22%3A%22Mesquita%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jacquelynn%22%2C%22lastName%22%3A%22Morrissey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ernesto%20Curty%22%2C%22lastName%22%3A%22da%20Costa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jorn%22%2C%22lastName%22%3A%22Hulsmann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Karis%22%2C%22lastName%22%3A%22Tang-Quan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yutao%22%2C%22lastName%22%3A%22Xi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Po-Feng%22%2C%22lastName%22%3A%22Lee%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Luiz%20C.%22%2C%22lastName%22%3A%22Sampaio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Doris%20A.%22%2C%22lastName%22%3A%22Taylor%22%7D%5D%2C%22abstractNote%22%3A%22The%20heart%20is%20a%20highly%20complex%2C%20multicellular%20solid%20organ%20with%20energy-demanding%20processes%20that%20require%20a%20dense%20vascular%20network%2C%20extensive%20cell-cell%20interactions%2C%20and%20extracellular%20matrix%20%28ECM%29-mediated%20crosstalk%20among%20heterogeneous%20cell%20populations.%20Here%2C%20we%20describe%20the%20regeneration%20of%20left%20ventricular%20%28LV%29%20wall%20using%20decellularized%20whole%20rabbit%20heart%20scaffolds%20recellularized%20exclusively%20with%20human%20induced%20pluripotent%20stem%20cell-derived%20endothelial%20cells%2C%20cardiomyocytes%2C%20and%20other%20cardiac%20cell%20types.%20Cells%20were%20sequentially%20delivered%20to%20the%20scaffold%20using%20an%20optimized%20endothelial%20cell%3Acardiomyocyte%20media.%20Macroscopic%20assessment%20after%2060%20days%20showed%20that%20the%20LV%20wall%20of%20recellularized%20hearts%20was%20anatomically%20restored%20to%20full%20thickness%20from%20base%20to%20apex%20and%20endocardium%20to%20epicardium.%20Histologic%20analysis%20of%20the%20recellularized%20LV%20wall%20revealed%20a%20heterogeneous%20pool%20of%20cardiac%20cells%20containing%20aligned%20cardiac%20troponin%20T-positive%20cells%20in%20close%20contact%20with%20ECM%3B%20vessels%20varied%20from%20large%20artery-like%2C%20surrounded%20by%20smooth%20muscle%20actin%2B%20cells%2C%20to%20capillary-like.%20Vessel%20patency%20was%20demonstrated%20after%20perfusion%20of%20recellularized%20hearts%20transplanted%20into%20the%20femoral%20artery%20bed%20of%20a%20pig.%20The%20construct%20exhibited%20visible%20beating%20and%20responded%20to%20chronotropic%20drug%20administration.%20These%20results%20demonstrate%20the%20ability%20to%20tissue%20engineer%20a%20vascularized%2C%20full-thickness%20LV%20wall%20with%20an%20unparalleled%20level%20of%20microanatomical%20organization%20and%20multicellular%20composition%2C%20using%20decellularized%20ECM%20and%20human%20cardiomyocytes%2C%20endothelial%20cells%2C%20and%20other%20cardiac%20cell%20types.%20STATEMENT%20OF%20SIGNIFICANCE%3A%20Decellularized%20extracellular%20matrix%20%28ECM%29%20is%20a%20bioactive%20template%20for%20tissue%20engineering%2C%20but%20recellularizing%20acellular%20whole%20heart%20scaffolds%20is%20challenging.%20Here%2C%20we%20successfully%20revascularized%20and%20repopulated%20a%20large%2C%20full-thickness%20portion%20of%20a%20ventricle%20using%20human%20induced%20pluripotent%20stem%20cell-derived%20endothelial%20and%20cardiac%20cells.%20At%2060%20days%2C%20histologic%20studies%20showed%20that%20the%20microanatomical%20organization%20and%20cellular%20composition%20of%20this%20region%20was%20similar%20to%20that%20of%20the%20native%20heart.%20The%20recellularized%20heart%20showed%20visible%20beating%20and%20responded%20appropriately%20to%20heartbeat-altering%20drugs.%20Vessels%20surrounded%20by%20smooth%20muscle%20cells%20and%20endothelial%20cells%20supported%20blood%20flow%20through%20the%20vessels%20of%20a%20recellularized%20heart%20that%20was%20surgically%20connected%20to%20a%20pig%20femoral%20artery.%20These%20findings%20move%20this%20approach%20closer%20to%20the%20possibility%20of%20clinical%20translation.%22%2C%22date%22%3A%222021-12-20%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.actbio.2021.12.016%22%2C%22ISSN%22%3A%221878-7568%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%224VC6SC4L%22%2C%22MJL5PY3A%22%2C%22PXVTG4ES%22%2C%22N9FF4AWY%22%2C%2235JIYMYN%22%2C%22IEPEFS59%22%5D%2C%22dateModified%22%3A%222022-11-15T19%3A37%3A32Z%22%7D%7D%2C%7B%22key%22%3A%22CPXTU9DW%22%2C%22library%22%3A%7B%22id%22%3A4862227%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Daoud%20et%20al.%22%2C%22parsedDate%22%3A%222021-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BDaoud%2C%20D.%2C%20Chacon%20Alberty%2C%20L.%2C%20Wei%2C%20Q.%20et%20al.%20%282021%29.%20Incidence%20of%20primary%20graft%20dysfunction%20is%20higher%20according%20to%20the%20new%20ISHLT%202016%20guidelines%20and%20correlates%20with%20clinical%20and%20molecular%20risk%20factors.%20%26lt%3Bi%26gt%3BJ%20Thorac%20Dis%26lt%3B%5C%2Fi%26gt%3B%20%26lt%3Bi%26gt%3B13%26lt%3B%5C%2Fi%26gt%3B%2C%203426%26%23x2013%3B3442.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.21037%5C%2Fjtd-20-3564%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.21037%5C%2Fjtd-20-3564%26lt%3B%5C%2Fa%26gt%3B.%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Incidence%20of%20primary%20graft%20dysfunction%20is%20higher%20according%20to%20the%20new%20ISHLT%202016%20guidelines%20and%20correlates%20with%20clinical%20and%20molecular%20risk%20factors%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daoud%22%2C%22lastName%22%3A%22Daoud%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lourdes%22%2C%22lastName%22%3A%22Chacon%20Alberty%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Qi%22%2C%22lastName%22%3A%22Wei%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camila%22%2C%22lastName%22%3A%22Hochman%20Mendez%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Muhammad%20Hassan%20Masood%22%2C%22lastName%22%3A%22Virk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jonathan%22%2C%22lastName%22%3A%22Mase%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Jindra%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthew%22%2C%22lastName%22%3A%22Cusick%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hyewon%22%2C%22lastName%22%3A%22Choi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Natalie%22%2C%22lastName%22%3A%22Debolske%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Luiz%20C.%22%2C%22lastName%22%3A%22Sampaio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Doris%20A.%22%2C%22lastName%22%3A%22Taylor%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gabriel%22%2C%22lastName%22%3A%22Loor%22%7D%5D%2C%22abstractNote%22%3A%22Background%3A%20Primary%20graft%20dysfunction%20%28PGD%29%20is%20the%20most%20important%20determinant%20of%20morbidity%20and%20mortality%20after%20lung%20transplantation%2C%20but%20its%20definition%20has%20evolved%20over%20the%20past%20decade.%20The%20implications%20of%20this%20refinement%20in%20clinical%20definition%20have%20not%20been%20evaluated.%20In%20this%20single-center%20study%2C%20we%20compared%20PGD%20incidence%2C%20risk%20factors%2C%20and%20outcomes%20using%20the%202005%20and%20the%20updated-2016%20International%20Society%20of%20Heart%20and%20Lung%20Transplantation%20guidelines%20for%20PGD%20grading%20in%20lung%20transplant%20patients.%5CnMethods%3A%20In%20this%20retrospective%20study%2C%20we%20extracted%20data%20from%20the%20medical%20records%20of%20127%20patients%20who%20underwent%20lung%20transplantation%20between%201%5C%2F1%5C%2F2016-12%5C%2F31%5C%2F2018.%20PGD%20was%20defined%20as%20PGD3%20present%20at%2048%20and%5C%2For%2072%20hours%20post-reperfusion.%20We%20used%20the%202005%20and%20the%20updated%202016%20guidelines%20to%20assess%20clinical%20risk%20factors%2C%20outcomes%2C%20and%20baseline%20biomarkers%20for%20PGD.%5CnResults%3A%20On%20the%20basis%20of%20the%202016%20and%202005%20guidelines%2C%20we%20identified%20PGD%20in%2037%25%20and%2026%25%20of%20patients%2C%20respectively.%20PGD%20was%20significantly%20associated%20with%20extracorporeal%20life%20support%2C%20large%20body%20mass%20index%2C%20and%20restrictive%20lung%20disease%20using%20the%202016%20but%20not%20the%202005%20guidelines.%20Based%20on%20the%202016%20guidelines%2C%20pretransplant%20levels%20of%20several%20biomarkers%20were%20associated%20with%20PGD%3B%20using%20the%202005%20guidelines%2C%20only%20increased%20interleukin-2%20levels%20were%20significantly%20associated%20with%20PGD.%20No%20preoperative%20biomarkers%20were%20associated%20with%20PGD%20using%20either%20guidelines%20after%20adjusting%20for%20clinical%20variables.%20Postoperative%20morbidity%20and%201-year%20mortality%20were%20similar%20regardless%20of%20guidelines%20used.%5CnConclusions%3A%20Our%20findings%20suggest%20that%20refinements%20in%20the%20PGD%20scoring%20system%20have%20improved%20the%20detection%20of%20graft%20injury%20and%20associated%20risk%20factors%20without%20changing%20its%20ability%20to%20predict%20postoperative%20morbidity%20and%20mortality.%22%2C%22date%22%3A%22June%202021%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.21037%5C%2Fjtd-20-3564%22%2C%22ISSN%22%3A%222072-1439%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22GNSKWJNU%22%2C%22MJL5PY3A%22%2C%22UDYH8SFB%22%5D%2C%22dateModified%22%3A%222021-08-05T18%3A53%3A12Z%22%7D%7D%5D%7D
Chacon-Alberty, L., Fernandez, R., Jindra, P. et al. (2023). Primary graft dysfunction in lung transplantation: a review of mechanisms and future applications. Transplantation. https://doi.org/10.1097/TP.0000000000004503.
Mesquita, F. C. P., Leite, E. S., Morrissey, J. et al. (2022). Polymerized laminin-521: a feasible substrate for expanding induced pluripotent stem cells at a low protein concentration. Cells 11, 3955. https://doi.org/10.3390/cells11243955.
Chacon-Alberty, L., Ye, S., Elsenousi, A. et al. (2022). Effect of intraoperative support mode on circulating inflammatory biomarkers after lung transplantation surgery. Artif Organs. https://doi.org/10.1111/aor.14474.
Guan, Y.-S., Ershad, F., Rao, Z. et al. (2022). Elastic electronics based on micromesh-structured rubbery semiconductor films. Nat Electron. https://doi.org/10.1038/s41928-022-00874-z.
Chacon-Alberty, L., Kanchi, R. S., Ye, S. et al. (2022). Plasma protein biomarkers for primary graft dysfunction after lung transplantation: a single-center cohort analysis. Sci Rep 12, 16137. https://doi.org/10.1038/s41598-022-20085-y.
Morrissey, J., Mesquita, F. C. P., Chacon-Alberty, L. et al. (2022). Mining the mesenchymal stromal cell secretome in patients with chronic left ventricular dysfunction. Cells 11, 2092. https://doi.org/10.3390/cells11132092.
Chacon-Alberty, L., Ye, S., Daoud, D. et al. (2021). Analysis of sex-based differences in clinical and molecular responses to ischemia reperfusion after lung transplantation. Respir Res 22, 318. https://doi.org/10.1186/s12931-021-01900-y.
Mesquita, F. C. P., Morrissey, J., Monnerat, G. et al. (2021). Decellularized extracellular matrix powder accelerates metabolic maturation at early stages of cardiac differentiation in human induced pluripotent stem cell-derived cardiomyocytes. Cells Tissues Organs. https://doi.org/10.1159/000521580.
Hochman-Mendez, C., Mesquita, F. C. P., Morrissey, J. et al. (2021). Restoring anatomical complexity of a left ventricle wall as a step toward bioengineering a human heart with human induced pluripotent stem cell-derived cardiac cells. Acta Biomater, S1742-7061(21)00823–0. https://doi.org/10.1016/j.actbio.2021.12.016.
Daoud, D., Chacon Alberty, L., Wei, Q. et al. (2021). Incidence of primary graft dysfunction is higher according to the new ISHLT 2016 guidelines and correlates with clinical and molecular risk factors. J Thorac Dis 13, 3426–3442. https://doi.org/10.21037/jtd-20-3564.
Recent News
Researchers at The Texas Heart Institute Pioneer Light-Responsive and Wireless Cardiac Stimulation Technologies
Researchers at The Texas Heart Institute (THI)—Camila Hochman-Mendez, Fernanda C. Paccola Mesquita, Ernesto Curty da Costa, Angel Moctezuma-Ramirez, and Abdelmotagaly...
Celebrating Women in Science at The Texas Heart Institute
As the world celebrates the International Day of Women and Girls in Science, a day dedicated to recognizing the invaluable...
The Texas Heart Institute Biorepository Demonstrates Excellence in Sample Quality with New Publication
A Study Validates THI’s Biorepository as a Global Leader in Long-Term Sample Preservation and Quality Assurance The Texas Heart Institute...
